Transgenic
outline
Microinjection
DNA purification
Sample
preparation for microinjection
Isolation
of DNA from mouse tail biopsies
neo
Primers
Timeline
for transgenic mouse analysis
BAC
DNA purification for transgenic mouse production
Genotyping
transgenic mice by PCR
beta-globin
Primers
Timeline
for generation of ES cell-derived mice
Preparation
of copy standards for PCR genotyping
lacZ
Primers
beta
galactosidase Staining
Transgenic
animals
Microinjection
of single cells in culture
DNA
purification for transgene microinjection
DNA
ultrapurification
DNA
preparation for microinjection or electroporation
Vasectomy
of mouse
1-cell
embryo transfer into pseudopregnant recipient female mouse
b-Galactosidase
solution assay
Lac-Z
detection in tail biopsies