Protein
Methods - US Dept. Commerce
Golgi-Vesicles
preparation from pea etiolated hypocotyls - Munoz
Transport
of UDP-[3H]-Glc to Golgi-Vesicles - Munoz
Rapid
protein prep
Rapid
yeast protein prep for SDS-PAGE & Western
Making
extracts for Westerns
Whole
cell extract using glass beads for western analysis
Yeast
lysates for Westerns
Protein
extraction from S. cerevisiae
Rapid
yeast protein prep
TCA
extraction of proteins
Nuclear
Extract (Small Scale)
Protein
Isolation "Yaffe-Schatz"
Pol
III whole cell yeast extract
Whole
cell extracts
Sucrose
gradient protocol
Osmotic
Shock Procedures - Hancock Lab
Preparation
of PGEX bacterial Lysates
Quantitation
of Epitope-tagged GPCR's by Biotin Labeling
Thrombin
cleavage of GST fused protein
Oxidase
Test
Biotinylation
of pGEX proteins
Flow
Cytometry Assay to measure Internalization of GPCR's
Protein
and LPS Stains
Serum
starving Her14 cells prior to EGF stimulation
Cytokine
Bioassays - eBioscience
Cytokine
Expression Profile - eBioscience
Purification
of Fibrinogen - HMS
Isolation
of Fish Fibronectin from Fish Fibrinogen
Isolation
of Plasminogen - HMS
Labeling
Microtubules - Molecular Dynamics Inc.
Em
observations of microsomes - William H. Heidcamp
Large
Scale Tubulin Preparation - Timothy Mitchison
The
Hill Reaction - Dr. WH Heidcamp
Preparation
of BMS Extract - Paulsson
Lacrimal
Experimental Protocol
Purification
of Endotoxin Free mAbs - Stephen Cobbold
HeLa
Cell Nuclei Preparation - John Garland
Extract
Preparation - Brent Graveley
Yeast
Nuclear Extract (Small Scale) - Hahn Lab.
Protein
Mass Spectrometry Protocols - PROWL
Kunxin
and Tamara Tryptic or Chymotryptic Peptide Mapping Protocol- BM Sefton
Peptide
map prediction - BM Sefton
Preparation
Of Peptide-KLH Conjugates for Immunization - TJ Mitchison
Extraction
& Electrophoresis of Histones - William H. Heidcamp
Protein
staining
TCA
precipitation
GST-fusion
protein expression and purification
Factor
Xa Cleavage of GST
Thrombin
Cleavage - Univ. Melbourne
GST-fusion
protein injection into rabbits
B-PER
Bacterial Protein extraction reagent
Preparation
of Protein-A -Rabbit antisera-immunoaffinity sepharose column
SDS/Native
PAGE
His-tag
protein elution method choice - Pierre Rodrigues
Native
purification of His-tagged Proteins from Sf9 cells
Preparation
of Immunoaffinity Column