Overview
Assays for protein purification and proteomics work - provided by Amos Heckendorf
Assays & Specific activity & Initial fractionation
Ion exchange - Dialysis & Concentration
Gel filtration, Affinity & Hydrophobic resins
Running the experiment & Resolving peaks
Purification steps
Making an affinity column
DEAE column preparation
DEAE column - FPLC
Affinity chromatography
Column chromatography for antibody conjugations - Gel filtration
Protein purification
Chelating or affinity tags
Protein Precipitation
Ammonium Sulfate Precipitation
Protein Precipitation Method - Gimila Lab.
TCA Precipitation with DOC - Waters Lab.
TCA protein precipitation - Hahn Lab.
His-tag protein purification
Protein isolation
SN-peptide receptor
Purification of GST-fused proteins
Preparation of immunoaffinity column
Actin purification - G150 column
Purification of skeletal muscle myosin
Lysozyme
Selective enzyme purification by affinity chromatography - Paper
Extraction of tyrosinase
Aldolase purification
Purification of Tailspike I - Jonathan King
Porin F Purification - Hancock Lab
Extraction of Tyrosinase - William H. Heidcamp
His-tag protein purification under denaturing conditions
Extraction and Electrophoresis of Histones
Preparation of Immunoaffinity Column
Isolation of Microtubules - Bovine Brain
Native purification of His-tagged Proteins from Sf9 cells
Preparing, isolating & characterizing ribosomes
Subcellular Fractionation of proteins
CHO Centrosome Prep - Timothy Mitchison
DNA purification by HPLC - Cynthia Wolberger
Affinity purification of antibodies from crude serum
Purification of Endotoxin Free mAbs - Stephen Cobbold
LPS Isolation - Hancock Lab
General Protocol for Affinity Purification of Antibodies from Serum
Purification of GST Fused Proteins
Actin Purification from Nonmuscle Sources - John Cooper
Affinity Purified Monospecific Antibodies to Gel-Purified Proteins
Preparation of Protein For Micro-sequencing - Larry Simpson
Actin Prep from Muscle - John Cooper
Actin Purification - G150 Column
Lipid Extraction