Human Cell Culture Methods - WUSTL
Aseptic Technique - Nanci Donacki
Culturing Peripheral Blood Lymphocytes
Preparation of Lymphoblastoid Cell Lines for Long Term Storage
Recommended working medium, trypsin volume & cell inoculation density
Useful Numbers for Cell Culture - Invitrogen (PDF file)
Routine Culturing of ES Cells - Peter MacCallum Cancer Institute
Surface Areas of Cell Culture Vessels
Blastocyst Transfer
Plastic dishes for growing cells
Low Speed Separaton of Cells - WH. Heidcamp
Maintenance of Cell Culture - Nanci Donacki
Adaptation of Cells to Serum Free Medium
Lymphocyte Transformation - Donis-Keller lab.
Cell Suspension Culture Methods - Gimila's lab.
Transfer of Eukaryote Suspension Cultures
Culture of BEND (Bovine Endometrial) Cells
Culture of endometrial explants
Cloning by Limiting Dilution - Nanci Donacki
Decontamination of Cultures with Antibiotics
Culturing of Trophoblast Stem Cell Lines - Janet Rossant
Removal of Yeast Contamination from Lymphoblast Cultures
Troubleshooting : Cell Culture - Invitrogen


Use of a Hemacytometer - Hansen
Hemacytometer Reference Guide - Becton Dickinson
Eukaryote Growth Dynamics - WH. Heidcamp
Cell Counts Using a Hemacytometer - Rosalie Veile
Cell Counting - Sefton lab.
Viable Cell Counts Using Trypan Blue
Viability Cell Count - WH. Heidcamp


Non-enzymatic Methods for Cell Harvesting - NUNC
Trypsinizing ES Cells
Preparation of Lymphocyte Cell Pellet for Storage - Donis-Keller lab.
Trypsinizing cells - Sefton lab.
Dissociation of Cells from Culture Vessels - Invitrogen
Dissociation of cells from Primary Tissue
Trypsinization of Adherent Cells - Suprya Jayadev