Animal ID:
RB2209/2210
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Host:
Rabbit
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Reactivity:
Human, Mouse
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Buffers:
Purified rabbit polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column and eluted out with both high and low pH buffers and neutralized immediately after elution then followed by dialysis against PBS.
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Storage:
Maintain refrigerated at 2-8ºC for up to 6 months. For long term storage store at -20ºC. Avoid repeated freeze-thaw cycles.
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Application:
Tested by peptide-specific ELISA (1:1,000).
WB (1:100 ~1:500), IHC (1:50 ~1:100)
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Immunogen:
KLH conjugated synthetic peptide comprised of amino acids 639 - 654 [SEEFELNDEKAATRMA] of the human ATP-binding cassette sub-family B (MDR/TAP) member 4 (ABCB4) protein.
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Accession number:
NM_000443
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Descrption:
The ATP-binding cassette sub-family B (MDR/TAP) member 4 (ABCB4) protein is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White) and ABCB4 is a member of the MDR/TAP subfamily. ABC proteins transport various molecules across extra- and intra-cellular membranes and members of the MDR/TAP subfamily are involved in multidrug resistance, lipid transportation as well as antigen presentation. ABCB4 biological function has not yet been fully determined; however, it may involve transport of phospholipids from liver hepatocytes into the bile. In man, ABCB4 alterations can cause familial intrahepatic cholestasis during pregnancy and progressive familial intrahepatic cholestasis (type 3). The ABCB4 gene encodes 3 different isoforms ( A, B and C). Protein isoform B contains a 7-amino acid in-frame insertion, as compared to the predominant isoform A, whereas isoform C contains a 47-amino acid in-frame deletion, as compared to isoform A. All three protein isoforms are seen by this antibody.
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Alternative Name(s):
ATP-binding cassette sub-family B (MDR/TAP) member 4, MDR3, PGY3, ABC21, MDR2/3, PFIC-3, multiple drug resistance 3 protein, P glycoprotein 3, ABCB4
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References:
- Jacquemin E:Mullenbach R, Linton KJ, Wiltshire S, Weerasekera N, Chambers J, Elias E, Higgins CF, Johnston DG, McCarthy MI, Williamson C: ABCB4 gene sequence variation in women with intrahepatic cholestasis of pregnancy. J. Med. Genet. 40(5): e70 (2003).
- Jacquemin E: Role of multidrug resistance 3 deficiency in pediatric and adult liver disease: one gene for three diseases. Semin. Liver Dis. 21(4): 551-562 (2001).
- Smit JJ, Schinkel AH, Oude Elferink RP, Groen AK, Wagenaar E, van Deemter L, Mol CA, Ottenhoff R, van der Lugt NM, van Roon MA, et al.: Homozygous disruption of the murine mdr2 P-glycoprotein gene leads to a complete absence of phospholipid from bile and to liver disease. Cell 75(3): 451-462 (1993).
- Smit JJ, Mol CA, van Deemter L, Wagenaar E, Schinkel AH, Borst P: Structure of the human MDR3 gene and physical mapping of the human MDR locus.J. Biol. Chem. 266(8): 5303-5310 (1991).
- Ruetz S, Gros P: The human mdr3 gene encodes a novel P-glycoprotein homologue and gives rise to alternatively spliced mRNAs in liver. EMBO J. 6(11): 3325-3331 (1987).
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For Research Use Only. Not for Diagnostic or Therapeutic Use.
Purchase does not include or carry any right to resell or transfer this product either as a stand-alone product or as a component of another product. Any use of this product other than the permitted use without the express written authorization of Orbigen, Inc. is strictly prohibited
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