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Choose Baculovirus Expression Services from Orbigen -
Highest Yield in the Market! | |
Baculovirus Expression Custom ServicesOrbigen's Custom Baculovirus Protein Expression Service offers the fastest and most cost-effective way for you to take advantage of the baculovirus expression technology.
Benefits of Protein Expression with Baculovirus:
- Eukaryotic post-translational modification
- Proper protein folding and function
- High expression levels
- Easy scale up with high-density suspension culture
- Safety
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Expression of GFP Using Baculovirus |
Orbigen's Advantages:
- Proprietary expression technology for up to 6-fold yield enhancement
- Experienced technical staff with more than 15 years of experience
- Modularized and customized service
- Competitive price to fit your budget
- Fast turnaround time
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Contact our Custom Service Representative today for more information or to schedule your project.
Phone: 1-866-672-4436 (US Toll-free) OR 1-858-362-2028 Option 1
Fax: 1-858-362-2030
E-mail: custom@orbigen.com
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Available Services:
| Service |
Description |
Cat. No. |
Price |
| Baculovirus Expression Full Service
[More Info] |
From cloned transfer vector to 500 ml high-titer, ready-to-use baculovirus stock. Including expression optimization in 3 cell lines with different infection conditions. |
BCS-10001 |
$3,800 |
| Baculovirus Expression Basic Service
[More Info] |
From cloned transfer vector to 30 ml high-titer, ready-to-use baculovirus stock. |
BCS-10002 |
$1,750 |
| Subcloning into Baculovirus Transfer Vector
[More Info] |
Subclone into a transfer vector using PCR cloning or restriction digest. |
BCS-10006 |
$999 |
| Amplification of Recombinant Baculovirus Stock
[More Info] |
Amplification of high-titer baculovirus stock into specified volumes. |
BCS-10003 |
$600 per 500 ml* |
| Baculovirus Expression Optimization
[More Info] |
Determine optimized expression condition using three different insect cell lines and two infection conditions. |
BCS-10004 |
$1,850 |
| Baculovirus Protein Production
[More Info] |
Insect cell expression at the capacity of up to 500 liters per week. |
BCS-10005 |
$600 per liter* |
| *Inquire for volume discount if more than 10 liters. |
Baculovirus Expression Full Service
| Catalog No.: |
BCS-10001 |
| Price (USD): |
$3,800 |
| Timeline: |
3~4 weeks |
| Required Materials: |
20 µg of recombinant baculovirus transfer plasmid containing gene of interest. |
| Deliverables: |
| Phase 1: |
3 cell pellets for evaluation of baculovirus to be used; |
| Phase 2: |
18 sets of cell pellets (including 6 negative controls) and media for evaluation of insect host and expression conditions; |
| Phase 3: |
500 ml of high-titer recombinant baculovirus stock solution ready to express the protein of interest. | | Service Description:
| Project Stage |
Description |
| Phase 1
Generation, Identification, and Evaluation of Baculovirus Recombinants |
Cotransfection of recombinant baculovirus transfer vector containing gene of interest with 3 different linearized Baculovirus DNAs. Recombinant clones will be identified and amplified. The customer will receive 3 cell pellets derived from the amplification for evaluation. The customer will choose the best recombinant baculovirus for the protein expression. |
| Phase 2
Expression Optimization |
Amplification of the selected recombinant baculovirus stock to a high-titer stock of more than 1 x 108 pfu per ml. The high-titer recombinant baculovirus stock will be used to infect three insect cell lines (Sf21, Sf9, and T.ni) with or without booster virus and cells are harvested at two different time points. The customer will choose the best insect host and infection conditions. |
| Phase 3
Amplification of High-titer Recombinant Baculovirus Stock |
Amplification of the selected recombinant baculovirus and titer determination of the final high-titer stock by end-point dilution. The customer will receive 500 ml of high-titer recombinant baculovirus stock as final product. |
Baculovirus Expression Basic Service
| Catalog No.: |
BCS-10002 |
| Price (USD): |
$1,750 |
| Timeline: |
2~3 weeks |
| Required Materials: |
20 µg of recombinant baculovirus transfer plasmid containing gene of interest. |
| Deliverables: |
| Phase 1: |
3 cell pellets for evaluation of baculovirus to be used; |
| Phase 2: |
30 ml of high-titer recombinant baculovirus stock solution ready to express the protein of interest. | | Service Description:
| Project Stage |
Description |
| Phase 1
Generation, Identification, and Evaluation of Baculovirus Recombinants |
Cotransfection of recombinant baculovirus transfer vector containing gene of interest with 3 different linearized Baculovirus DNAs. Recombinant clones will be identified and amplified. The customer will receive 3 cell pellets derived from the amplification for evaluation. The customer will choose the best recombinant baculovirus for the protein expression |
| Phase 2
Amplification of High-titer Recombinant Baculovirus Stock |
Amplification of the selected recombinant baculovirus and titer determination of the final high-titer stock by end-point dilution.
The customer will receive 30 ml of high-titer recombinant baculovirus stock as final product. |
Amplification of Recombinant Baculovirus Stock
| Catalog No.: |
BCS-10003 |
| Price (USD): |
$600 for each 500 ml |
| Timeline: |
2~3 weeks |
| Required Materials: |
At least 5 ml of recombinant baculovirus stock. |
| Deliverables: |
Specified quantity (in ml) of high-titer recombinant baculovirus stock solution ready to express the protein of interest. |
Service Description:
- Amplification of the chosen recombinant baculovirus;
- Titration of final high-titer stock by end point dilution
Baculovirus Expression Optimization Service
| Catalog No.: |
BCS-10004 |
| Price (USD): |
$1,850 |
| Timeline: |
2~3 weeks |
| Required Materials: |
At least 5-10 ml of recombinant baculovirus stock. |
| Deliverables: |
18 sets of cell pellets and media for evaluation of protein yield. |
Service Description:
- Amplification of the chosen recombinant baculovirus to a high-titer stock of more than 1 x 108 pfu per ml;
- Infection of three insect cell lines (Sf21, Sf9, and T.ni using the high-titer stock and with or without booster virus;
- Cells are harvested at two different time points.
Table. Conditions for Expression Optimization
| Cell Lines Used |
Sf9, T.ni, Sf21 |
| Booster Virus |
Presence and Absence |
| MOI |
10 |
| Time Points |
72 hours and 96 hours |
Protein Production Service
| Catalog No.: |
BCS-10005 |
| Price (USD): |
$600 for each liter of culture |
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Discount is available if you require 10 liters or more |
| Timeline: |
2~3 weeks (1 - 5 liters), |
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4~5 weeks (5 - 20 liters), |
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8~10 weeks (20 liters and above) |
| Required Materials: |
At least 100 ml of high-titer (>108 pfu) recombinant baculovirus stock.
Optimized protein expression conditions are determined by previous evaluation project. |
| Deliverables: |
Cell pellets ready for protein purification or in the case of a secreted recombinant protein, the customer will receive the supernatant |
Service Description:
- Amplification of recombinant baculovirus stock for protein production;
- Expression of recombinant protein under optimized conditions.
Subcloning into Baculovirus Transfer Vector
| Catalog No.: |
BCS-10006 |
| Price (USD): |
$999 |
| Timeline: |
2~3 weeks |
| Required Material*: |
5 µg of plasmid containing gene of interest.
Complete vector sequence or map. |
| Deliverables: |
20 µg of ready-to-use recombinant baculovirus transfer plasmid containing the gene of interest |
Service Description:
- Isolating the gene of interest from a supplied vector and subcloning it into a baculovirus transfer vector;
- Verification of recombinant baculovirus transfer vector by restriction mapping and sequencing of junctions;
- Preparation and purification of recombinant vector suitable for co- transfection with baculovirus DNA.
*Note: Customers will be responsible for the cost incurred in the subcloning service if the sequence of the supplied plasmid does not correspond with the sequence provided.
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