Description:
- Up to 6 fold yield increase compared to other baculovirus expression systems
- Improved protein quality through enhanced disulfide bond formation
- Higher recombination efficiency
Sapphire™ Baculovirus DNA was specifically designed for efficient recombination with a polyhedrin promoter-based baculovirus transfer vector. The Sapphire™ DNA contains a lethal deletion of ORF1629, which can only result in viable viral particles if rescued by homologous recombination with a polyhedrin promoter. This design leads to virtually 100% recombination efficiency and significantly simplifies identification of recombinant virus. In addition, the disulfide isomerases gene was inserted at the p10 locus of the virus to ensure proper protein disulfide bond formation. The Sapphire™ Baculovirus DNA is compatible with most of the commercial polyhedrin-based transfer vectors.
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Content:
The Sapphireż Baculovirus DNA is supplied at 0.1 µg/µl concentration.
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Storage:
Store at + 4˚C. Be sure NOT to freeze the viral DNA. The Sapphireż Baculovirus DNA is stable for 6 months when stored properly.
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