Animal ID:
RB0651-0652
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Host:
Rabbit
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Reactivity:
human
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Buffers:
Purified rabbit polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column and eluted out with both high and low pH buffers and neutralized immediately after elution then followed by dialysis against PBS.
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Storage:
Maintain refrigerated at 2-8ºC for up to 6 months. For long term storage store at -20ºC. Avoid repeated freeze-thaw cycles.
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Application:
Tested by peptide-specific ELISA (1:1,000).
WB (1:100 ~1:500)
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Immunogen:
KLH conjugated synthetic peptide comprised of amino acids 16 - 32 [CDRSVPPARALLKRRRS] of the human "sentrin/SUMO-specific protease 2, N-terminus (SENP2)" protein.
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Accession number:
NM_021627
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Description:
Covalent attachment of one protein to another is one of the more prominent posttranslational modifications in respects to size and ubiquity ¿ to which eukaryotic proteins are subject. Ubiquitin is the most familiar of the protein modifiers and its activation and transfer to target proteins has been studied for over two decades. Recently a new group of ubiquitin-like (Ubl) proteins have come to light. One of the most intriguing of them is SUMO (small ubiquitin-like modifier) also known as Sentrin. Sentrin-1 is a protein of 101 residues that is distantly related to ubiquitin and another ubiquitin-like protein, NEDD8. Sentrin/SUMO proteins have been described in many vertebrates. They have been shown to bind and regulate mammalian SP-RINGs (such as Mdm2, PIAS and PML), RanGAP1, RanBP2, p53, p73, HIPK2, TEL, c-Jun, Fas, Daxx, TNFRI, Topo-I, Topo-II, WRN, Sp100, IkB-alpha, Androgen receptor, GLUT1/4, Drosophila Ttk69, Dorsal, CaMK, yeast septins, and viral CMV-IE1/2, EBV-BZLF1 and HPV/BPV-E1 expression. Thus, SUMO may be involved in stabilizing target proteins and/or influence their subcellular localization. Sentrin/SUMO-specific protease 2 (SENP2) is involved in destabilizing and degradation of specific protein complexes. Its association with the nuclear pore plays an important negative role in the regulation of SENP2.
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Alternative Name(s):
AXAM2, SMT3IP2, KIAA1331, Sentrin-specific protease 2; SMT3-specific isopeptidase 2, SENP2
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References:
- Zhang H, Saitoh H, Matunis MJ: Enzymes of the SUMO modification pathway localize to filaments of the nuclear pore complex. Mol. Cell. Biol. 22(18): 6498-6508 (2002).
- Hang J, Dasso M: Association of the human SUMO-1 protease SENP2 with the nuclear pore. J. Biol. Chem. 277(22): 19961-19966 (2002).
- Nishida T, Kaneko F, Kitagawa M, Yasuda H: Characterization of a novel mammalian SUMO-1/Smt3-specific isopeptidase, a homologue of rat axam, which is an axin-binding protein promoting beta-catenin degradation. J. Biol. Chem. 276(42): 39060-39066 (2001).
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For Research Use Only. Not for Diagnostic or Therapeutic Use.
Purchase does not include or carry any right to resell or transfer this product either as a stand-alone product or as a component of another product. Any use of this product other than the permitted use without the express written authorization of Orbigen, Inc. is strictly prohibited
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