Animal ID:
RB0545-0546
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Host:
Rabbit
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Reactivity:
human
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Buffers:
Purified rabbit polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column and eluted out with both high and low pH buffers and neutralized immediately after elution then followed by dialysis against PBS.
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Storage:
Maintain refrigerated at 2-8ºC for up to 6 months. For long term storage store at -20ºC. Avoid repeated freeze-thaw cycles.
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Application:
Tested by peptide-specific ELISA (1:1,000).
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Immunogen:
KLH conjugated synthetic peptide comprised of amino acids 389 - 405 [KGEPSLPEKDEDHALSY] of the human non-receptor type 1 protein tyrosine phosphatase (PTPN1) protein.
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Accession number:
NM_002827
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Description:
PTP1B (PTPN1)is a non-receptor type 1 protein tyrosine phosphatase and the founding member of the protein tyrosine phosphatase (PTP) family, which was isolated and identified based on its enzymatic activity and amino acid sequence. PTPs catalyze the hydrolysis of the phosphate monoesters specifically on tyrosine residues. Members of the PTP family share a highly conserved catalytic motif, which is essential for the catalytic activity. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP has been shown to act as a negative regulator of insulin signaling by dephosphorylating the phosphotryosine residues of insulin receptor kinase. This PTP was also reported to dephosphorylate epidermal growth factor receptor kinase, as well as JAK2 and TYK2 kinases, which implicated the role of this PTP in cell growth control, and cell response to interferon stimulation.
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Alternative Name(s):
protein tyrosine phosphatase, non-receptor type 1, PTP1B, PTP-1B, protein tyrosine phophatase 1B, non-receptor tyrosine phophatase 1, placental protein tyrosine phophatase
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References:
- Yigzaw Y, Poppleton HM, Sreejayan N, Hassid A, Patel TB: Protein-tyrosine phosphatase-1B (PTP1B) mediates the anti-migratory actions of Sprouty. J. Biol. Chem. 278(1): 284-288 (2003).
- Myers MP, Andersen JN, Cheng A, Tremblay ML, Horvath CM, Parisien JP, Salmeen A, Barford D, Tonks NK: TYK2 and JAK2 are substrates of protein-tyrosine phosphatase 1B. J. Biol. Chem. 276(51): 47771-47774 (2001).
- Charbonneau H, Tonks NK, Kumar S, Diltz CD, Harrylock M, Cool DE, Krebs EG, Fischer EH, Walsh KA:Human placenta protein-tyrosine-phosphatase: amino acid sequence and relationship to a family of receptor-like proteins. Proc. Natl. Acad. Sci. U S A. 86(14): 5252-5256 (1989).
- Dadke S, Kusari A, Kusari J: Phosphorylation and activation of protein tyrosine phosphatase (PTP) 1B by insulin receptor. Mol. Cell. Biochem. 221(1-2): 147-154 (2001).
- Brown-Shimer S, Johnson KA, Lawrence JB, Johnson C, Bruskin A, Green NR, Hill DE: Molecular cloning and chromosome mapping of the human gene encoding protein phosphotyrosyl phosphatase 1B. Proc. Natl. Acad. Sci. U S A. 87(13): 5148-5152 (1990).
- Chernoff J, Schievella AR, Jost CA, Erikson RL, Neel BG: Cloning of a cDNA for a major human protein-tyrosine-phosphatase. Proc. Natl. Acad. Sci. U S A. 87(7): 2735-2739 (1990).
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For Research Use Only. Not for Diagnostic or Therapeutic Use.
Purchase does not include or carry any right to resell or transfer this product either as a stand-alone product or as a component of another product. Any use of this product other than the permitted use without the express written authorization of Orbigen, Inc. is strictly prohibited
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