Animal ID:
RB2129/2130
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Host:
Rabbit
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Reactivity:
Human
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Buffers:
Purified rabbit polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column and eluted out with both high and low pH buffers and neutralized immediately after elution then followed by dialysis against PBS.
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Storage:
Maintain refrigerated at 2-8ºC for up to 6 months. For long term storage store at -20ºC. Avoid repeated freeze-thaw cycles.
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Application:
Tested by peptide-specific ELISA (1:1,000).
WB (1:100 ~1:500), IHC (1:50 ~1:100)
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Immunogen:
KLH conjugated synthetic peptide comprised of amino acids 201 - 218 [DDAEVEAILNSGARGYSA] of the human melanoma antigen family H 1 (MAGEH1) protein.
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Accession number:
NM_014061
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Description:
The human MAGE genes are expressed in a wide variety of tumors but not in normal cells, with the exception of the male germ cells, placenta, and, possibly, cells of the developing embryo. Aberrant MAGE expression lead to tumor-specific antigens recognized on a human melanoma by autologous cytolytic T lymphocytes. The human MAGE genes are located on the X chromosome, in four clusters denoted MAGE-A, B, C, and D mapping at q28, p21.3, q26, and p11.2, respectively. MAGE-H1 is thought to be involved in apoptosis. Genes hMAGE-B10, hMAGE-B16, hMAGE-B17, and hMAGE-C4 displayed the expression pattern characteristic of the MAGE-A, -B, and -C genes, i.e., silent in all normal tissues with the exception of testis. In addition, hMAGE-C4 is expressed in a minor proportion of tumor samples, suggesting that it could potentially encode tumor-specific antigens. By contrast, all hMAGE-D, hMAGE-E, hMAGE-F, hMAGE-G, and hMAGE-H genes are expressed at various levels in many normal tissues, a ubiquitous expression pattern resembling that reported for hMAGE-D1 and -D2.
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Alternative Name(s):
Member H1 of the melanoma antigen family, melanoma-associated antigen H1, APR-1, restin, MAGEH1
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References:
- Chomez P, De Backer O, Bertrand M, De Plaen E, Boon T, Lucas S: An overview of the MAGE gene family with the identification of all human members of the family. Cancer Res. 61(14): 5544-5551 (2001).
- Zhu F, Yan W, Zhao ZL, Chai YB, Lu F, Wang Q, Peng WD, Yang AG, Wang CJ: Improved PCR-based subtractive hybridization strategy for cloning differentially expressed genes. Biotechniques 29(2): 310-313 (2000).
- Lucas S, De Plaen E, Boon T: MAGE-B5, MAGE-B6, MAGE-C2, and MAGE-C3: four new members of the MAGE family with tumor-specific expression. Int. J. Cancer 87(1): 55-60 (2000).
- Pold M, Zhou J, Chen GL, Hall JM, Vescio RA, Berenson JR: Identification of a new, unorthodox member of the MAGE gene family. Genomics 59(2): 161-167 (1999).
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For Research Use Only. Not for Diagnostic or Therapeutic Use.
Purchase does not include or carry any right to resell or transfer this product either as a stand-alone product or as a component of another product. Any use of this product other than the permitted use without the express written authorization of Orbigen, Inc. is strictly prohibited
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