Animal ID:
RB2135/2136
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Host:
Rabbit
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Reactivity:
Human
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Buffers:
Purified rabbit polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column and eluted out with both high and low pH buffers and neutralized immediately after elution then followed by dialysis against PBS.
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Storage:
Maintain refrigerated at 2-8ºC for up to 6 months. For long term storage store at -20ºC. Avoid repeated freeze-thaw cycles.
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Application:
Tested by peptide-specific ELISA (1:1,000).
WB (1:100 ~1:500), IHC (1:50 ~1:100)
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Immunogen:
KLH conjugated synthetic peptide comprised of amino acids 441 - 456 [NLRPSPNLRPSPNSRA] of the human melanoma antigen family D 1 (MAGED1) protein.
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Accession number:
NM_006986
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Description:
The human MAGE genes are expressed in a wide variety of tumors but not in normal cells, with the exception of the male germ cells, placenta, and, possibly, cells of the developing embryo. Aberrant MAGE expression lead to tumor-specific antigens recognized on a human melanoma by autologous cytolytic T lymphocytes. The human MAGE genes are located on the X chromosome, in four clusters denoted MAGE-A, B, C, and D mapping at q28, p21.3, q26, and p11.2, respectively. Although MAGE-D1 shares strong homology with other members of the MAGE family, but in contrary to these, MAGE-D1 is expressed in almost all normal adult tissues and has been demonstrated to interact with the p75 neurotrophin receptor and to facilitate nerve growth factor-dependent apoptosis. MAGE-D1 also interacts with members of the Dlx/Msx homeodomain family and to regulate the transcriptional function of Dlx5.
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Alternative Name(s):
Member D1 of the melanoma antigen family, melanoma-associated antigen D1, NRAGE, DLXIN-1, neurotrophin receptor-interacting MAGE homolog, MAGED1
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References:
- Sasaki A, Masuda Y, Iwai K, Ikeda K, Watanabe K: A RING finger protein Praja1 regulates Dlx5-dependent transcription through its ubiquitin ligase activity for the Dlx/Msx-interacting MAGE/Necdin family protein, Dlxin-1. J. Biol. Chem. 277(25): 22541-22546 (2002).
- Masuda Y, Sasaki A, Shibuya H, Ueno N, Ikeda K, Watanabe K: Dlxin-1, a novel protein that binds Dlx5 and regulates its transcriptional function. J. Biol. Chem. 276(7): 5331-5338 (2001).
- Kubu CJ, Goldhawk DG, Barker PA, Verdi JM: Identification of the translational initiation codon in human MAGED1. Genomics 70(1): 150-152 (2000).
- Salehi AH, Roux PP, Kubu CJ, Zeindler C, Bhakar A, Tannis LL, Verdi JM, Barker PA: NRAGE, a novel MAGE protein, interacts with the p75 neurotrophin receptor and facilitates nerve growth factor-dependent apoptosis. Neuron 27(2): 279-288 (2000).
- Pold M, Zhou J, Chen GL, Hall JM, Vescio RA, Berenson JR: Identification of a new, unorthodox member of the MAGE gene family. Genomics 59(2): 161-167 (1999).
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For Research Use Only. Not for Diagnostic or Therapeutic Use.
Purchase does not include or carry any right to resell or transfer this product either as a stand-alone product or as a component of another product. Any use of this product other than the permitted use without the express written authorization of Orbigen, Inc. is strictly prohibited
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