Animal ID:
RB2105/2106
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Host:
Rabbit
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Reactivity:
Human
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Buffers:
Purified rabbit polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column and eluted out with both high and low pH buffers and neutralized immediately after elution then followed by dialysis against PBS.
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Storage:
Maintain refrigerated at 2-8ºC for up to 6 months. For long term storage store at -20ºC. Avoid repeated freeze-thaw cycles.
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Application:
Tested by peptide-specific ELISA (1:1,000).
IHC (1:50 ~1:100)
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Immunogen:
KLH conjugated synthetic peptide comprised of amino acids 47 - 61 [GATIQKKSAGRSRSA] of the human melanoma antigen family B 3 (MAGEB3) protein.
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Accession number:
NM_002365
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Description:
The human MAGE genes are expressed in a wide variety of tumors but not in normal cells, with the exception of the male germ cells, placenta, and, possibly, cells of the developing embryo. Genes of the MAGE family direct the expression of tumor-specific antigens recognized on a human melanoma by autologous cytolytic T lymphocytes. The MAGE genes are located on the X chromosome, in four clusters denoted MAGE-A, B, C, and D mapping at q28, p21.3, q26, and p11.2, respectively. Twelve closely related MAGE-A genes are located in the Xq28 region which share 60-98% nucleotide identity in their coding region. The Xp21.3 region contains four MAGE-B genes (MAGE-B1, MAGE-B2, MAGE-B3, and MAGE-B4). The coding regions of these genes share 66-81% nucleotide identity and show 45-63% identity with MAGE-A genes. Like the MAGE-A, the MAGE-B genes are silent in normal tissues with the exception of testis and are expressed in a significant fraction of tumors of various histological types. The function of these genes remains unknown.
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Alternative Name(s):
Member B3 of the melanoma antigen family, melanoma-associated antigen B3, MAGEB3
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References:
- Lurquin C, De Smet C, Brasseur F, Muscatelli F, Martelange V, De Plaen E, Brasseur R, Monaco AP, Boon T: Two members of the human MAGEB gene family located in Xp21.3 are expressed in tumors of various histological origins. Genomics 46(3): 397-408 (1997).
- Muscatelli F, Walker AP, De Plaen E, Stafford AN, Monaco AP: Isolation and characterization of a MAGE gene family in the Xp21.3 region. Proc. Natl. Acad. Sci. U S A. 92(11): 4987-4991 (1995).
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For Research Use Only. Not for Diagnostic or Therapeutic Use.
Purchase does not include or carry any right to resell or transfer this product either as a stand-alone product or as a component of another product. Any use of this product other than the permitted use without the express written authorization of Orbigen, Inc. is strictly prohibited
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