Animal ID:
RB2193/2194
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Host:
Rabbit
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Reactivity:
Human
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Buffers:
Purified rabbit polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column and eluted out with both high and low pH buffers and neutralized immediately after elution then followed by dialysis against PBS.
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Storage:
Maintain refrigerated at 2-8ºC for up to 6 months. For long term storage store at -20ºC. Avoid repeated freeze-thaw cycles.
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Application:
Tested by peptide-specific ELISA (1:1,000).
WB (1:100 ~1:500), IHC (1:50 ~1:100)
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Immunogen:
KLH conjugated synthetic peptide comprised of amino acids 96 - 108 [RMFPDLESEFQAA] of the human melanoma antigen family A 2 (MAGEA2) protein.
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Accession number:
NM_005361
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Description:
The human MAGE genes are expressed in a wide variety of tumors but not in normal cells, with the exception of the male germ cells, placenta, and, possibly, cells of the developing embryo. Genes of the MAGE family direct the expression of tumor-specific antigens recognized on a human melanoma by autologous cytolytic T lymphocytes. The MAGE genes are located on the X chromosome, in four clusters denoted MAGE-A, B, C, and D mapping at q28, p21.3, q26, and p11.2, respectively. Twelve closely related MAGE-A genes including MAGE-A2 are located in the Xq28 region. They share 60-98% nucleotide identity in their coding region. The function of these genes remains unknown. The MAGE-A2 gene is expressed in a higher proportion of melanoma tumors than MAGE-A1. It is also expressed in many small-cell lung carcinomas and other lung tumors, laryngeal tumors, and sarcomas. No MAGE-A1 and MAGE-A2 gene expression was found in a large panel of healthy adult tissues, with the exception of testis.
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Alternative Name(s):
Member A2 of the melanoma antigen family, melanoma antigen family A2, MAGEA2
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References:
- Rogner UC, Wilke K, Steck E, Korn B, Poustka A: The melanoma antigen gene (MAGE) family is clustered in the chromosomal band Xq28. Genomics 29(3): 725-731 (1995).
- De Smet C, Lurquin C, van der Bruggen P, De Plaen E, Brasseur F, Boon T: Sequence and expression pattern of the human MAGE2 gene. Immunogenetics 39(2): 121-129 (1994).
- van der Bruggen P, Traversari C, Chomez P, Lurquin C, De Plaen E, Van den Eynde B, Knuth A, Boon T:A gene encoding an antigen recognized by cytolytic T lymphocytes on a human melanoma. Science 254(5038): 1643-1647 (1991).
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For Research Use Only. Not for Diagnostic or Therapeutic Use.
Purchase does not include or carry any right to resell or transfer this product either as a stand-alone product or as a component of another product. Any use of this product other than the permitted use without the express written authorization of Orbigen, Inc. is strictly prohibited
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