Animal ID:
RB0611-0612
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Host:
Rabbit
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Reactivity:
human
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Buffers:
Purified rabbit polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column and eluted out with both high and low pH buffers and neutralized immediately after elution then followed by dialysis against PBS.
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Storage:
Maintain refrigerated at 2-8ºC for up to 6 months. For long term storage store at -20ºC. Avoid repeated freeze-thaw cycles.
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Application:
Tested by peptide-specific ELISA (1:1,000).
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Immunogen:
KLH conjugated synthetic peptide comprised of amino acids 318 - 335 [CLSTLKDLDTSDRKEDVL] of the human Bloom syndrome (BLM) protein.
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Accession number:
NM_000057
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Description:
The Bloom syndrome protein (BLM) is related to the RecQ subset of DExH box-containing DNA helicases and has both DNA-stimulated ATPase and ATP-dependent DNA helicase activities. Bloom syndrome-causing mutations either alter or delete helicase motifs and abolish the 3'-5' helicase activity. The normal BLM protein suppresses inappropriate recombination events. BLM protein is mainly localized in the nucleus and binds to the human mismatch repair protein MLH1 and the telomere-binding protein TRF2. BLM is also required for correct relocalization of the RAD50/MRE11/NBS1 complex after replication fork arrest and colocalizes with the Werner syndrome protein. Additionally, BLM interacts and cooperates with p53 in regulation of transcription and cell growth control. BLM heterozygosity largely increases the risk of colorectal cancer.
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Alternative Name(s):
Bloom syndrome protein, BS, RECQ2, RECQL2, RECQL3
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References:
- Gruber SB, Ellis NA, Scott KK, Almog R, Kolachana P, Bonner JD, Kirchhoff T, Tomsho LP, Nafa K, Pierce H, Low M, Satagopan J, Rennert H, Huang H, Greenson JK, Groden J, Rapaport B, Shia J, Johnson S, Gregersen PK, Harris CC, Boyd J, Offit K: BLM heterozygosity and the risk of colorectal cancer. Science 297(5589): 2013 (2002).
- Opresko PL, von Kobbe C, Laine JP, Harrigan J, Hickson ID, Bohr VA: Telomere-binding protein TRF2 binds to and stimulates the Werner and Bloom syndrome helicases. J. Biol. Chem. 277(43): 41110-41119 (2002).
- Karow JK, Chakraverty RK, Hickson ID: The Bloom's syndrome gene product is a 3'-5' DNA helicase. J. Biol. Chem. 272(49): 30611-30614 (1997).
- Ellis NA, Groden J, Ye TZ, Straughen J, Lennon DJ, Ciocci S, Proytcheva M, German J: The Bloom's syndrome gene product is homologous to RecQ helicases. Cell 83(4): 655-666 (1995).
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For Research Use Only. Not for Diagnostic or Therapeutic Use.
Purchase does not include or carry any right to resell or transfer this product either as a stand-alone product or as a component of another product. Any use of this product other than the permitted use without the express written authorization of Orbigen, Inc. is strictly prohibited
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