Animal ID:
RB1975/1976
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Host:
Rabbit
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Reactivity:
Human
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Buffers:
Purified rabbit polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column and eluted out with both high and low pH buffers and neutralized immediately after elution then followed by dialysis against PBS.
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Storage:
Maintain refrigerated at 2-8ºC for up to 6 months. For long term storage store at -20ºC. Avoid repeated freeze-thaw cycles.
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Application:
Tested by peptide-specific ELISA (1:1,000).
IHC (1:50 ~1:100)
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Immunogen:
KLH conjugated synthetic peptide comprised of amino acids 695 - 709 [SLWGSLKPKRLGAHT] of the human "amyloid beta (A4) protein-binding family B member 1 (APBB1, Fe65)" protein.
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Accession number:
NM_001164
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Description:
Amyloid beta (A4) protein-binding family B member 1 (APBB1, Fe65) is an adaptor protein localized in the nucleus that interacts with the Alzheimer's disease amyloid precursor protein (APP), the transcription factor CP2/LSF/LBP1 and the low-density lipoprotein receptor-related protein. APP functions as a cytosolic anchoring site that can prevent the gene product's nuclear translocation. APBB1 seems to play an important role in the pathogenesis of Alzheimer's disease. Presumably, APBB1 is blocking cell cycle progression by downregulating the thymidylate synthase expression. The APBB1 gene codes for at least two isoforms (E9 and delta-E9) of which both are recognized by the antibody. The E9 isoform is exclusively expressed in neurons, whereas delta-E9 is widely expressed in all non-neuronal cells but not in differentiated neurons.
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Alternative Name(s):
amyloid beta (A4) protein-binding family B member 1, RIR, FE65, Fe65, MGC9072, stat-like adaptor protein FE65a2
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References:
- Bruni P, Minopoli G, Brancaccio T, Napolitano M, Faraonio R, Zambrano N, Hansen U, Russo T: Fe65, a ligand of the Alzheimer's beta-amyloid precursor protein, blocks cell cycle progression by down-regulating thymidylate synthase expression. J. Biol. Chem. 277(38): 35481-35488 (2002).
- Hu Q, Cool BH, Wang B, Hearn MG, Martin GM: A candidate molecular mechanism for the association of an intronic polymorphism of FE65 with resistance to very late onset dementia of the Alzheimer type. Hum. Mol. Genet. 11(4): 465-475 (2002).
- Bressler SL, Gray MD, Sopher BL, Hu Q, Hearn MG, Pham DG, Dinulos MB, Fukuchi K, Sisodia SS, Miller MA, Disteche CM, Martin GM: cDNA cloning and chromosome mapping of the human Fe65 gene: interaction of the conserved cytoplasmic domains of the human beta-amyloid precursor protein and its homologues with the mouse Fe65 protein. Hum. Mol. Genet. 5(10): 1589-1598 (1996).
- McLoughlin DM, Miller CC: The intracellular cytoplasmic domain of the Alzheimer's disease amyloid precursor protein interacts with phosphotyrosine-binding domain proteins in the yeast two-hybrid system. FEBS Lett. 397(2-3): 197-200 (1996).
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For Research Use Only. Not for Diagnostic or Therapeutic Use.
Purchase does not include or carry any right to resell or transfer this product either as a stand-alone product or as a component of another product. Any use of this product other than the permitted use without the express written authorization of Orbigen, Inc. is strictly prohibited
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