Animal ID:
RB1857-1858
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Host:
Rabbit
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Reactivity:
human
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Buffers:
Purified rabbit polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column and eluted out with both high and low pH buffers and neutralized immediately after elution then followed by dialysis against PBS.
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Storage:
Maintain refrigerated at 2-8ºC for up to 6 months. For long term storage store at -20ºC. Avoid repeated freeze-thaw cycles.
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Application:
Tested by peptide-specific ELISA (1:1,000).
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Immunogen:
KLH conjugated synthetic peptide comprised of amino acids 20 - 34 [GASHASLIPETGKKK] of the human bone morphogenetic protein 4 (BMP4) protein.
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Accession number:
NM_001202
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Description:
Bone morphogenetic proteins (BMPs) were originally identified by an ability of demineralized bone extract to induce endochondral osteogenesis in vivo in an extraskeletal site. All BMPs except BMP1 are members of the transforming growth factor-beta superfamily of secreted growth and differentiation factors and they signal through bone morphogenetic protein receptors (BMPRs), membrane-bound serine/threonine kinases. BMP proteins are synthesized as prepropeptides, cleaved, and then processed into dimeric proteins. Bone morphogenetic protein 4 (BMP4) plays an important role in the onset of endochondral bone formation in humans. A reduction in expression of BMP4 has been associated with a variety of bone diseases, including the heritable disorder fibrodysplasia (myositis) ossificans progressiva. BMP4 may also be involved in specification of tooth identity as experiments with developing mouse embryos suggest. BMP4 can activate Dkk1 (dickkopf 1) but only when it concomitantly induces apoptosis. Implanted recombinant human BMP4 beads abolish Dkk1 transcription in chick limb buds and mouse embryo cells. The BMP4 gene encodes for two different protein isoforms (1 and 2). Protein isoform 1 utilizes exons 1, 3, 4 and 5, resulting in the longest transcript. Protein isoform 2 utilizes exons 1, 3, 4 and 5 but uses an alternate splice acceptor site on exon 1, resulting in a shorter transcript compared to isoform 1.
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Alternative Name(s):
bone morphogenetic protein 4, ZYME, BMP2B, BMP2B1, bone morphogenetic protein 2B
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References:
- Grotewold L, Ruther U: The Wnt antagonist Dickkopf-1 is regulated by Bmp signaling and c-Jun and modulates programmed cell death. EMBO J. 21(5): 966-975 (2002).
- Tucker AS, Matthews KL, Sharpe PT: Transformation of tooth type induced by inhibition of BMP signaling. Science 282(5391): 1136-1138 (1998).
- Shore EM, Xu M, Shah PB, Janoff HB, Hahn GV, Deardorff MA, Sovinsky L, Spinner NB, Zasloff MA, Wozney JM, Kaplan FS: The human bone morphogenetic protein 4 (BMP-4) gene: molecular structure and transcriptional regulation. Calcif. Tissue Int. 63(3): 221-229 (1998).
- van den Wijngaard A, van Kraay M, van Zoelen EJ, Olijve W, Boersma CJ: Genomic organization of the human bone morphogenetic protein-4 gene: molecular basis for multiple transcripts. Biochem. Biophys. Res. Commun. 219(3): 789-794 (1996).
- Oida S, Iimura T, Maruoka Y, Takeda K, Sasaki S: Cloning and sequence of bone morphogenetic protein 4 (BMP-4) from a human placental cDNA library. DNA Seq. 5(5): 273-275 (1995).
- Wozney JM, Rosen V, Celeste AJ, Mitsock LM, Whitters MJ, Kriz RW, Hewick RM, Wang EA: Novel regulators of bone formation: molecular clones and activities. Science 242(4885): 1528-1534 (1988).
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For Research Use Only. Not for Diagnostic or Therapeutic Use.
Purchase does not include or carry any right to resell or transfer this product either as a stand-alone product or as a component of another product. Any use of this product other than the permitted use without the express written authorization of Orbigen, Inc. is strictly prohibited
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