Animal ID:
RB1091/RB1092
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Host:
Rabbit
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Reactivity:
Human
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Buffers:
Purified rabbit polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column and eluted out with both high and low pH buffers and neutralized immediately after elution then followed by dialysis against PBS.
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Storage:
Maintain refrigerated at 2-8ºC for up to 6 months. For long term storage store at -20ºC. Avoid repeated freeze-thaw cycles.
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Application:
Tested by peptide-specific ELISA (1:1,000).
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Immunogen:
KLH conjugated synthetic peptide comprised of amino acids 471 - 483 [APDILAKSPQSTR] of the human ATPase alpha polypeptide Cu++ transporting (ATP7B) protein.
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Accession number:
NM_000053
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Description:
A major obstacle in the treatment of human solid carcinomas is the intrinsic/acquired resistance to cisplatin-based chemotherapy. Copper-transporting P-type adenosine triphosphatase (ATP7B) has been reported to be associated with cisplatin resistance in vitro. ATP7B is overexpressed in human solid carcinomas such as breast, gastric and oral squamous cell carcinomas. ATP7B expression has an influential effect on some subsets of patients with cisplatin-treated carcinomas. ATP7B mutation is well-known as a cause of Wilson's disease. In addition, the six copper-binding domain and ATP-binding domain of ATP7B are important for the transportation of metals.
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Alternative Name(s):
ATPase, Cu++ transporting, beta polypeptide, Wilson disease protein, WND
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References:
- Kanzaki A, Nakayama K, Miyashita H, Shirata S, Nitta Y, Oubu M, Higashimoto M, Mutoh M, Mori S, Konno S, Ogawa K, Toi M, Takebayashi Y: Mutation analysis of copper-transporting P-type adenosine triphosphatase (ATP7B) in human solid carcinomas.Anticancer Res. 23(2C): 1913-1915 (2003).
- Lutsenko S, Tsivkovskii R, Walker JM: Functional properties of the human copper-transporting ATPase ATP7B (the Wilson's disease protein) and regulation by metallochaperone Atox1. Ann. N Y Acad. Sci. 986: 204-211 (2003).
- Higashimoto M, Kanzaki A, Shimakawa T, Konno S, Naritaka Y, Nitta Y, Mori S, Shirata S, Yoshida A, Terada K, Sugiyama T, Ogawa K, Takebayashi Y: Expression of copper-transporting P-type adenosine triphosphatase in human esophageal carcinoma. Int. J. Mol. Med. 11(3): 337-341 (2003).
- Harada M, Kumemura H, Sakisaka S, Shishido S, Taniguchi E, Kawaguchi T, Hanada S, Koga H, Kumashiro R, Ueno T, Suganuma T, Furuta K, Namba M, Sugiyama T, Sata M: Wilson disease protein ATP7B is localized in the late endosomes in a polarized human hepatocyte cell line. Int. J. Mol. Med. 11(3): 293-298 (2003).
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For Research Use Only. Not for Diagnostic or Therapeutic Use.
Purchase does not include or carry any right to resell or transfer this product either as a stand-alone product or as a component of another product. Any use of this product other than the permitted use without the express written authorization of Orbigen, Inc. is strictly prohibited
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