Animal ID:
RB1817-1818
|
Host:
Rabbit
|
Reactivity:
Human
|
Buffers:
Purified rabbit polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column and eluted out with both high and low pH buffers and neutralized immediately after elution then followed by dialysis against PBS.
|
Storage:
Maintain refrigerated at 2-8ºC for up to 6 months. For long term storage store at -20ºC. Avoid repeated freeze-thaw cycles.
|
Application:
Tested by peptide-specific ELISA (1:1,000).
IHC (1:50 ~1:100)
|
|
|
Immunogen:
KLH conjugated synthetic peptide comprised of amino acids 689 - 703 [GQDLYSRLVQQRLMD] of the human ATP-binding cassette sub-family B (MDR/TAP) transporter 2 (TAP2) protein.
|
Accession number:
NM_000544
|
Description:
The membrane-associated protein ATP-binding cassette sub-family B (MDR/TAP) transporter 2 (TAP2) is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). TAP1 and TAP2 are two major histocompatibility complex (MHC) genes, located between HLA-DP and -DQ, whose products form a transporter molecule involved in endogenous antigen processing. TAP2 forms a heterodimer with ABCB2 in order to transport peptides from the cytoplasm to the endoplasmic reticulum where MHC class I molecules assemble. Polymorphic residues have been described in both TAP genes. In man, mutations in TAP2 cause bare lymphocyte syndrome (type 1). The TAP2 gene encodes two protein isoforms (1 and 2). Isoform 1 represents the predominant form of TAP2 and contains exon 11 and a longer 3' UTR, whereas isoform 2 lacks exon 11, has exon 10 spliced to exon 12 resulting in a translational frameshift, truncated protein, and a distinct 3' UTR, as compared to variant 1. Both protein isoforms differ in peptide selectivity and level of restoration of surface expression of MHC class I molecules. This antibody only recognizes TAP2 protein isoform 1.
|
Alternative Name(s):
ABC transporter TAP2, MHC 2, peptide supply factor 2, peptide transporter PSF2, antigen peptide transporter 2, TAP2
|
References:
- Saveanu L, Van Endert PM: Regulation of transporters associated with antigen processing (TAPs) by nucleotide binding to, and hydrolysis by, Walker consensus sequences. Adv. Exp. Med. Biol. 495: 79-82 (2001).
- Yan G, Shi L, Faustman D: Novel splicing of the human MHC-encoded peptide transporter confers unique properties. J. Immunol. 162(2): 852-859 (1999).
- Powis SH, Tonks S, Mockridge I, Kelly AP, Bodmer JG, Trowsdale J: Alleles and haplotypes of the MHC-encoded ABC transporters TAP1 and TAP2. Immunogenetics 37(5): 373-380 (1993).
- Bahram S, Arnold D, Bresnahan M, Strominger JL, Spies T: Two putative subunits of a peptide pump encoded in the human major histocompatibility complex class II region. Proc. Natl. Acad. Sci. U S A. 88(22): 10094-10098 (1991).
- Kelly A, Powis SH, Kerr LA, Mockridge I, Elliott T, Bastin J, Uchanska-Ziegler B, Ziegler A, Trowsdale J, Townsend A: Assembly and function of the two ABC transporter proteins encoded in the human major histocompatibility complex. Nature 355(6361): 641-644 (1992).
|
For Research Use Only. Not for Diagnostic or Therapeutic Use.
Purchase does not include or carry any right to resell or transfer this product either as a stand-alone product or as a component of another product. Any use of this product other than the permitted use without the express written authorization of Orbigen, Inc. is strictly prohibited
|
|
