Animal ID:
RB1827-1828
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Host:
Rabbit
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Reactivity:
Human
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Buffers:
Purified rabbit polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column and eluted out with both high and low pH buffers and neutralized immediately after elution then followed by dialysis against PBS.
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Storage:
Maintain refrigerated at 2-8ºC for up to 6 months. For long term storage store at -20ºC. Avoid repeated freeze-thaw cycles.
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Application:
Tested by peptide-specific ELISA (1:1,000).
WB (1:100 ~1:500), IHC (1:50 ~1:100)
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Immunogen:
KLH conjugated synthetic peptide comprised of amino acids 18 - 32 [NNSGKELSSHWRPKD] of the human endothelial differentiation sphingolipid G-protein-coupled receptor 4 (EDG4) protein.
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Accession number:
NM_004720
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Description:
The integral membrane protein endothelial differentiation sphingolipid G-protein-coupled receptor 4 (EDG4) is a lysophosphatidic acid (LPA) receptor from a group known as EDG receptors. These receptors are members of the G protein-coupled receptor superfamily. Utilized by LPA for cell signaling, EDG receptors transduce signals from LPA and S1P that stimulate ras- and rho-dependent cellular proliferation, enhance cellular survival, and suppress apoptosis and mediate diverse biologic functions such as platelet aggregation, smooth muscle contraction, inhibition of neuroblastoma cell differentiation, chemotaxis, and tumor cell invasion. EDG1, 3, and 5 bind sphingosine 1-phosphate (S1P), whereas Edg2 and 4 bind LPA. That high levels of LPA in plasma and ascitic fluid of patients with ovarian cancer correlate with widespread invasion suggested the importance of investigating expression and functions of Edg Rs in ovarian cancer cells as compared with nonmalignant ovarian surface epithelial cells. EDG4 contributes to Ca++ mobilization, a critical cellular response to LPA in cells, through association with Gi and Gq proteins.
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Alternative Name(s):
endothelial differentiation/lysophosphatidic acid G-protein-coupled receptor 4, LPA2, EDG-4, LPA receptor EDG4, G protein-coupled receptor, lysophosphatidic acid receptor EDG4
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References:
- Hama K, Bandoh K, Kakehi Y, Aoki J, Arai H: Lysophosphatidic acid (LPA) receptors are activated differentially by biological fluids: possible role of LPA-binding proteins in activation of LPA receptors. FEBS Lett. 523(1-3): 187-192 (2002).
- Bandoh K, Aoki J, Taira A, Tsujimoto M, Arai H, Inoue K: Lysophosphatidic acid (LPA) receptors of the EDG family are differentially activated by LPA species. Structure-activity relationship of cloned LPA receptors. FEBS Lett. 478(1-2): 159-165 (2000).
- Goetzl EJ, Dolezalova H, Kong Y, Hu YL, Jaffe RB, Kalli KR, Conover CA: Distinctive expression and functions of the type 4 endothelial differentiation gene-encoded G protein-coupled receptor for lysophosphatidic acid in ovarian cancer. Cancer Res. 59(20): 5370-5375 (1999).
- An S, Bleu T, Zheng Y, Goetzl EJ: Recombinant human G protein-coupled lysophosphatidic acid receptors mediate intracellular calcium mobilization. Mol. Pharmacol. 54(5): 881-888 (1998).
- An S, Bleu T, Hallmark OG, Goetzl EJ: Characterization of a novel subtype of human G protein-coupled receptor for lysophosphatidic acid. J. Biol. Chem. 273(14): 7906-7910 (1998).
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For Research Use Only. Not for Diagnostic or Therapeutic Use.
Purchase does not include or carry any right to resell or transfer this product either as a stand-alone product or as a component of another product. Any use of this product other than the permitted use without the express written authorization of Orbigen, Inc. is strictly prohibited
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